Header image with fluorescence microscopy image showing cells with supernumerary centrosomes

Geoffrey A. Charters MSc (Hons) PhD (Pathology)

Molecular oncopathologist

Introduction

Curriculum vitae

Biography

About me

Contact information

General

Intellectual

  • Extremely strong inductive reasoning skills that enable robust hypothesis generation: the extraction of the relatively sparse salient elements from a large body of often internally inconsistent information and the formulation of a consistent model from them, while simultaneously identifying areas of conflict between the model and the unincorporated data, and any significant missing elements.
  • Complementary deductive reasoning skills that allow the development of research or experimental methodologies to test or expand these theoretical models.
  • An ability to perceive patterns in systems that can facilitate understanding through the use of analogies drawn from unassociated fields.

Communication

  • Excellent written communication skills, with an ability to present complex material in a concise, precise, complete, consistent, clear, and orderly manner.
  • An ability to conceive and produce complex diagrammatic representations of systems to aid in their description.
  • Meticulous attention to details of spelling, grammar, and consistency of style.

Scientific

General

  • Routine operation of materials handling/measuring systems: pipettes, balances, centrifuges, pH meters, spectrophotometers, fluorometers etc.
  • Safe handling of toxic, mutagenic, corrosive, and biohazardous material.
  • Good basic understanding of statistical methods: linear regression, hypothesis testing.

Cellular and molecular biology

  • Familiarity with online tools and resources: Pubmed, OMIM, BLAST, Primer3, etc.
  • Preparation of routine cell culture solutions and reagents for experimental procedures.
  • Routine maintenance of human cells in serial passage culture.
  • Storage of and retrieval from cryogenic storage of viable human cells.
  • Phase contrast, fluorescence, and laser-scanning confocal microscopy.
  • Flow cytometric determination of ploidy and cell cycle phasing.
  • Flow cytometric determination of protein expression.
  • Isolation, purification, and concentration of high quality DNA, RNA, and protein.
  • Polymerase chain reaction design, optimisation, and execution.
  • Agarose and acrylamide gel electrophoresis of nucleic acids.
  • Acrylamide electrophoresis and Western blotting of proteins.
  • Preparation of PCR products for sequencing.
  • Analysis of DNA sequencing and microsatellite analysis results.
  • Restriction and ligation enzyme procedures.
  • Bacterial transfection of PCR products.

Computational

Applications

  • Highly proficient user of Apple Macintosh systems and standard software.
  • Extensive knowledge of Microsoft Office applications.
  • Good working knowledge of Adobe Photoshop.
  • Familiarity with a great many other applications and utilities.

Programming

  • Formerly, an expert with PASCAL and Macro-11 assembler for RSX11.
  • Fair competency with Visual Basic for Applications.
  • Some skill with Java, Objective-C, Javascript, DHTML, XHTML, PHP, JSP, MySQL.

Miscellaneous

  • Dual New Zealand/British citizenship.
  • Full N. Z. car driver licence.
  • Significant knowledge of: science and technology in general; 35 mm film SLR and DSLR photography; native N. Z. flora.
  • Excellent cook.